Journal: Food Science & Nutrition
Article Title: Nobiletin Ameliorates Skeletal Muscle Performance in D‐Galactose‐Induced Aging Mice by Boosting Aerobic Metabolism
doi: 10.1002/fsn3.71416
Figure Lengend Snippet: Nob ameliorated mitochondrial ultrastructure and function in D‐gal‐induced aging mice. (A) Mitochondrial ultrastructure, scale bar = 2 um or 500 μm, n = 3 mice/group. (B) Number of mitochondria. (C) Mitochondrial size. (D) Total cristae length per mitochondria area. (E) The activity of complex IV, n = 4 mice/group. (F) Total ATP production, n = 3. (G‐H) Western blot analysis of Mfn1, Mfn2, OPA1, Drp1 and β‐tubulin in D‐gal‐induced aging mice treated with vehicle and Nob, n = 3 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: The PGC‐1α monoclonal antibody (66369‐1‐Ig), NRF2 Rabbit mAb (16396‐1‐AP), SIRT1 polyclonal antibody (13161‐1‐AP), MFN1 Polyclonal antibody (13798‐1‐AP), OPA1 Polyclonal antibody (27733‐1‐AP), and Beta Tubulin Polyclonal antibody (10094‐1‐AP) were purchased from Proteintech (Wuhan, China).
Techniques: Activity Assay, Western Blot